Towards development of new technique for cryopreservation of date palm (phoenix dactylifera l.) in vitro grown cultures

Mohei El Din Solliman, Abdullatif A. Al-Khateeb, Heba Allah Mohasseb, Mohammed I. Aldaej, Suliman A. Al-Khateeb

Research output: Contribution to journalJournal articlepeer-review

Abstract

The method of cryopreservation was described as very simple with the potential application of a wide range of date palms. The undifferentiated tissue cultures have been successfully frozen by later freezing methods of renewed seedlings. Shoot tips of date palm were cultured on M S-medium containing 10 mg/1 2,4-D + 3mg/1 2iP. The em-bryogenic callus of about 65% of the water content caused by 20 minutes of air drying period recorded the highest percentage of survival and in vitro conversion to plants. Among the different types of sugars (sucrose) used as pretreatment agents in the pre-plant average, sucrose was the best for the persistence of cryogenic palm tree cultures. The highest survival rate (80%) and switch to plants (70%) were observed with 1 M sucrose. Cryopreservation of date palm embryogenic callus via encapsulation is a newly methods we trying to introduce to our lab and spread for others also. The encapsulation is a simple process that can be done inserting the prop- A gule singly into an alginate drop and then falling it into a complexing solution (20-35 min) to form calcium alginate capsules that are approximately 5 mm in diameter. The presence of nutritive elements into the capsule (artificial endosperm) has a function similar to endosperm of zygotic seeds. RAPD was extensively used to study the genetic stability of cryogenic tissue cultures from date palm. According to RAPD analysis, the seedlings derived from cryopreserved cultures were similar to those derived from untreated cultures, both of which were similar to the cultivated plants in the field.

Original languageEnglish
Pages (from-to)6256-6263
Number of pages8
JournalFresenius Environmental Bulletin
Volume28
Issue number8
StatePublished - 2019

Keywords

  • Cry cryopreservation
  • Date palm
  • Embryogenesis
  • Khalas
  • Rapd analysis
  • Vitrification

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