Role for Plant Thioredoxin in Cd 2+ Chelation

Moêz Smiri, Jamel El Ghoul

Research output: Contribution to journalJournal articlepeer-review

4 Scopus citations

Abstract

Despite the critical role of thioredoxin (Trx) in redox regulation, little is known of the function in seed germination, especially in the presence of heavy metal. Plant redox reactions are principally studied on adult plant organs and there is little information on the onset of redox regulation during seed germination. Regulation of the antioxidant defense system during seed germination during the transition from heterotrophic growth to photoautotrophic metabolism in seedlings was examined in pea (Pisum sativum L.) in the presence of cadmium (Cd). Properties of the Cd 2+-Trx interaction on the Trx from pea were examined to determine whether its binding affected Trx activity. When incubated with Cd in vitro the disulfide reductase activity of Trx o was inhibited. Disruption of the vicinal dithiol on Trx via oxidation to the disulfide (-S-S-) bridge protected against Cd and is consistent with a dithiol chelation mechanism. Thioredoxins are involved in defense and detoxification, antioxidant activity, and germination. Quantification of these proteins provides new insight for understanding the molecular bases of responses to heavy metal in seed during germination.

Original languageEnglish
Pages (from-to)93-105
Number of pages13
JournalInternational Journal of Vegetable Science
Volume18
Issue number1
DOIs
StatePublished - Jan 2012
Externally publishedYes

Keywords

  • Cadmium
  • Germination
  • Pisum sativum
  • Thioredoxins

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