New spectrophotometric methods for estimation of ethacridine lactate in bulk and pharmaceutical formulations using 1, 10-phenanthroline and folin ciocaltaeu reagent

Research output: Contribution to journalJournal articlepeer-review

Abstract

Ethacridine Lactate (EAL) is an antiseptic in 0.1% solutions. It is also used as an agent for inducing abortion. Two simple and sensitive spectrophotometric methods (Method A and Method B) were developed for the estimation of Ethacridine Lactate. Method A is based on the oxidative coupling of Ethacridine Lactate with 1, 10- phenanthroline in the presence of Fe(III) to form a chromophore with absorption maximum of 490nm. Method B is based on redox reaction of Ethacridine Lactate with Folin ciocaltaeu reagent to form a chromogen estimated at 600nm. Methods A and B obey Beer's law in the concentration range of 2 to 18 and 5 to 35 μg/ml respectively. Interference studies were conducted and it was found that the common excipients usually present in dosage forms do not interfere in the proposed methods. The optical characteristics, regression analysis data and precision of the methods were calculated. The accuracy of the methods was evaluated by estimating the amount of Ethacridine Lactate in previously analyzed samples to which known amounts of Ethacridine Lactate was spiked. The accuracy of the methods was also conformed by comparison of the results obtained by proposed and reference methods. The methods were validated for use in routine quality control of Ethacridine Lactate in pharmaceutical formulations.

Original languageEnglish
Pages (from-to)1799-1802
Number of pages4
JournalInternational Journal of ChemTech Research
Volume2
Issue number3
StatePublished - Jul 2010
Externally publishedYes

Keywords

  • 1,10-Phenanthroline
  • Development
  • Ethacridine lactate
  • Folin ciocaltaeu reagent
  • Spectrophotometry
  • Validation

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