Molecular characterization of tomato yellow leaf curl disease associated viruses in Saudi Arabia

K. Alhudiab, W. Alaraby, Adel Rezk

Research output: Contribution to journalJournal articlepeer-review

5 Scopus citations

Abstract

Tomato production in Saudi Arabia and anywhere is under constant threat of whitefly-transmitted begomoviruses that cause tomato yellow leaf curl disease (TYLCD). Tomato yellow leaf curl virus (TYLCV) that has a large number of strains affects tomato all over the world. Tomato samples with typical symptoms of TYLCD were collected from open fields and greenhouses from different locations of Hail and Qasim in Saudi Arabia. PCR was used to identify the pathogen using degenerate primers of Geminiviruses (AVcore and ACcore) and other specific primers. To determine exactly the virus strain, multiplex PCR was used with sets of specific primers for Tomato yellow leaf curl Almeria strain (TYLCV-Alm), Tomato yellow leaf curl virus Israel strain (TYLCV-IL), Tomato yellow leaf curl virus Mild strain (TYLCV-Mld) and Tomato yellow leaf curl Sardinia virus (TYLCSV). All tested samples showed negative with specific primers in multiplex-PCR, nevertheless, most of symptomatic tomato samples were positive with the use of degenerate primers. Notwithstanding, PCR products were cloned and sequenced as well as deposited in GenBank. Thus, the results showed that all obtained isolates were found to have >95% similarity with Tomato leaf curl virus isolates from Sudan and Yemen.

Original languageEnglish
Pages (from-to)192-203
Number of pages12
JournalInternational Journal of Virology
Volume10
Issue number3
DOIs
StatePublished - 2014

Keywords

  • Multiplex-PCR
  • Saudi Arabia
  • Tomato
  • TYLCV

Fingerprint

Dive into the research topics of 'Molecular characterization of tomato yellow leaf curl disease associated viruses in Saudi Arabia'. Together they form a unique fingerprint.

Cite this