Grape seed proanthocyanidin extract protects against carrageenan-induced lung inflammation in mice through reduction of pro-inflammatory markers and chemokine expressions

Sheikh Fayaz Ahmad, Khairy M.A. Zoheir, Hala E. Abdel-Hamied, Sabry M. Attia, Saleh A. Bakheet, Abdelkader E. Ashour, Adel R.A. Abd-Allah

Research output: Contribution to journalJournal articlepeer-review

30 Scopus citations

Abstract

Proanthocyanidins are the most abundant phenolic compounds and have been reported to exert anti-inflammatory actions. The aim of this study was to investigate the effects of grape seed proanthocyanidin extract (GSPE) in a mouse model of carrageenan-induced pleurisy. Following the induction of pleurisy using λ-carrageenan (Cg, 1 %), GSPE (25, 50 and 100 mg/kg) was administered per-oral (p.o.), and the glucocorticoid-induced tumour necrosis factor receptor (GITR), IL-17A expressing cells and other markers, such as cytokines (Th1/Th2 and Th17), were studied. We evaluate the effects of GSPE on the mRNA expression of pro-inflammatory and anti-inflammatory mediators. The results illustrated that the cell numbers of IL-17A and GITR expressing cells and the cytokine levels in Th1/Th17 cells were markedly increased in the Cg-group, whereas the cytokines produced by Th2 cells were significantly decreased in the same group. Treatment with GSPE reversed these effects. Histological examinations revealed anti-inflammatory effects of GSPE.

Original languageEnglish
Pages (from-to)500-511
Number of pages12
JournalInflammation
Volume37
Issue number2
DOIs
StatePublished - Apr 2014
Externally publishedYes

Keywords

  • carrageenan
  • cytokines
  • GITR
  • grape seed proanthocyanidin extract
  • lung
  • pleural exudate

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