Fisetin and telmisartan each alone or in low-dose combination alleviate OVA-induced food allergy in mice

Reem Elkholy, mohamed balaha, Noha El-Anwar, Samah Kandeel, Sabiha Hedya, Mohamed Nabih Abd-El Rahman

Research output: Contribution to journalJournal articlepeer-review

Abstract

Background: Food allergy (FA) is a worldwide health problem, affecting nearly 10% of all populations, with no prophylactic options or regulatory treatment available until now. Fisetin, a biologically active flavonoid, and telmisartan, the highly selective competitive AT1 receptor antagonist, recently exhibited potent anti-inflammatory and immunomodulatory activities. In the present study, we have evaluated the possible anti-inflammatory and immunomodulatory activities of fisetin and telmisartan each alone or in low-dose combination in a mouse model of FA. Methods: For induction of FA, eight-week-old BALB/c mice, sensitized by two ip injection of 50 μg ovalbumin (OVA) and 1 mg alum at day 0 and 7. Then, each mouse challenged with 10 mg OVA at days 14, 16, 18, and 21. On the 28 th day, the fifth challenge carried out by oral administration of 50 mg OVA. Either fisetin (1 or 3 mg/kg/d), telmisartan (1 or 3 mg/kg/d) or a combination of fisetin 1 mg/kg/d and telmisartan 1 mg/kg/d received orally from the 13 th day till 28 th day. In challenge days, the treatments received one-hour before the challenge. Results: Our data showed that fisetin and telmisartan each alone or in low-dose combination attenuated the anaphylactic manifestation, decreased blood eosinophilic count, serum OVA-specific IgE, and IL-4 levels, the intestinal total and degranulated mast cells count, and CD4 + immunohistochemical expression. Furthermore, they enhanced the serum IFN-γ level and abrogated the intestinal histopathological changes induced by OVA in mice. Conclusion: Either fisetin, telmisartan or their low-dose combination could be promising in the management of FA.

Original languageEnglish
Pages (from-to)330-337
Number of pages8
JournalPharmacological Reports
Volume71
Issue number2
DOIs
StatePublished - Apr 2019

Keywords

  • Fisetin
  • IFN-γ
  • IL-4
  • OVA-food allergy
  • Telmisartan

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