Escheriosome-mediated cytosolic delivery of PLK1-specific siRNA: Potential in treatment of liver cancer in BALB/c mice

Arun Chauhan, Swaleha Zubair, Ahmad Nadeem, Sajid Ansari, Mohammad Yunus Ansari, Owais Mohammad

Research output: Contribution to journalJournal articlepeer-review

13 Scopus citations

Abstract

Aim: In the present study, the anticancer efficacy of a novel escheriosome-based formulation of PLK1-specific siRNA was evaluated against liver cancer in BALB/c mice. Materials & methods: The escheriosome-based siRNA nanoparticles were prepared using lipids isolated from Escherichia coli. The escheriosomes were characterized for size, surface charge and stability. The anticancer potential of PLK1-specific siRNA formulation was ascertained on the basis of expression of pro-/anti-apoptotic factors and histopathological studies. Results: The escheriosome-entrapped siRNA was found to be released in surrounding milieu in a sustained manner. The nanoformulation was successful in modulating proapoptotic factors and eventually helped in better survival of the treated animals. Conclusion: Our data demonstrate the efficacy of systemically administered siRNA in the treatment of experimental liver cancer. This novel therapeutic strategy may be applicable to a broad range of cancers in patients with the obstinate form of the disease.

Original languageEnglish
Pages (from-to)407-420
Number of pages14
JournalNanomedicine
Volume9
Issue number4
DOIs
StatePublished - Mar 2014
Externally publishedYes

Keywords

  • apoptosis
  • DEN
  • escheriosome
  • liver cancer
  • nanoparticle
  • p53
  • PLK1
  • siRNA

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