Determination of As(III) and As(V) species in some natural water and food samples by solid-phase extraction on Streptococcus pyogenes immobilized on Sepabeads SP 70 and hydride generation atomic absorption spectrometry

Ozgur Dogan Uluozlu, Mustafa Tuzen, Durali Mendil, Mustafa Soylak

Research output: Contribution to journalJournal articlepeer-review

82 Scopus citations

Abstract

The speciation of arsenic(III) and arsenic(V) by using Streptococcus pyogenes immobilized on Sepabeads SP 70 resin has been investigated with solid-phase extraction method. The arsenic levels were determined hydride generation atomic absorption spectrometry (HGAAS) in sample solutions. The procedure presented based on quantitative recoveries of As(III) as >95%. Also the As(V) recoveries were obtained as <5% using the presented method. After reduction of As(V) by using KI and ascorbic acid and waiting 1. h later, the system was applied to determination of total arsenic. As(V) was found as the difference between the total As and As(III) content. Various experimental parameters such as pH, amount of microorganism, sample volume, etc. were investigated. The capacity of biosorbent for arsenic(III) was calculated as 7.3. mg/g. The preconcentration factor was found as 36. The relative standard deviation was calculated below 8%. Limit of detection was calculated as 13. ng/L. The validation of the presented procedure was tested by analysis of standard reference materials (NIST SRM 1568a Rice floor and GBW 07605 Tea) and obtained fairly compatible results. The procedure was also successfully applied to arsenic speciation and determination of some natural water and food samples.

Original languageEnglish
Pages (from-to)1393-1398
Number of pages6
JournalFood and Chemical Toxicology
Volume48
Issue number5
DOIs
StatePublished - May 2010
Externally publishedYes

Keywords

  • Arsenic
  • HGAAS
  • Sepabeads SP 70
  • Solid-phase extraction
  • Speciation
  • Streptococcus pyogenes

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