A green approach for simultaneous analysis of two natural hepatoprotective drugs in pure forms, capsules and human plasma using HPLC-UV method

Mohammed Gamal, Ali Hazim, Rehab M. Abdelfatah, Maimana A. Magdy

Research output: Contribution to journalJournal articlepeer-review

8 Scopus citations

Abstract

A green high performance liquid chromatographic method with ultra violet detector (HPLC-UV) is presented, for the first time, for the simultaneous estimation of two hepatoprotective drugs in capsules and spiked human plasma. The first component is a herbal supplement, namely silymarin (SR) and the second is vitamin E acetate (VE). The method utilized an isocratic elution using C8 column, a mobile phase consisting of methanol:acetonitrile:triethylamine:trifluoroacetic acid (85:15:0.2:0.1, by volume) at 1 mL/min flow rate and 210 nm as a measuring wavelength. All silymarin flavonolignans were measured together as a single peak. The method was evaluated for precision, selectivity, linearity, accuracy, LOQ, and LOD. SR and VE were detected in the linear range of 0.3–50 μg/mL and 0.1 to 50 μg/mL respectively. The Eco-scale score of the new HPLC method was 62 while it was 55 for the previous electrochemical method. Calibration curves had correlation coefficients of >0.999. The new method is better than the old electrochemical voltammetric method in terms of simplicity, greenness, wide linear range and analysis time.

Original languageEnglish
Article number104258
JournalMicrochemical Journal
Volume151
DOIs
StatePublished - Dec 2019

Keywords

  • Greenness
  • HPLC
  • Plasma
  • Silymarin
  • Validation
  • Vitamin E acetate

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